Acid pH-induced conformational changes in bovine liver rhodanese.
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چکیده
منابع مشابه
Acid pH-induced conformational changes in bovine liver rhodanese.
The enzyme rhodanese is greatly stabilized in the range pH 4-6, and samples at pH 5 are fully active after several days at 23 degrees C. This is very different from results at pH greater than 7, where there is significant loss of activity within 1 h. A pH-dependent conformational change occurs below pH 4 in a transition centered around pH 3.25 that leads slowly to inactive rhodanese at pH 3 (t ...
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The pigeon liver fatty acid synthetase complex (s:~,~ = 14.0 S) is inactivated and dissociated into half-molecular weight subunits (s”,,,, = 9.0 S) in the presence of low ionic strength buffers. The rates of inactivation and dissociation of the complex are dependent upon the ionic strength, pH, and temperature of the medium. At low ionic strengths (0.01 or less) and mildly alkaline pH (8.35), t...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1992
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)41798-x